Schal Lab Resources

Our lab maintains a small collection of pest, temperate, and tropical cockroach species, and several colonies of bed bugs, including various strains recently collected in apartments in various US locations. The Insectary within the Department of Entomology and Plant Pathology, housed in the Biological Resources Facility, also maintains other exotic insects for display and outreach activities. We welcome requests from non-profit organizations for cockroaches to be used in educational programs.

List of cockroach species in culture

Blaberidae

Blaberus atropos Gromphadorhina portentosa
Blaberus craniifer Leucophaea (Rhyparobia) maderae
Blaberus giganticus Nauphoeta cinerea
Blaptica dubia Pycnocelus indicus
Diploptera puncata Pycnocelus surinamensis
Eublaberus posticus Schultesia lampyridiformes

Blattidae

Periplaneta australasiae Periplaneta fuliginosa (collected locally)
Periplaneta americana (collected locally) Blatta orientalis (collected locally)

Ectobiidae

Blattella germanica (many strains) Parcoblatta latta (collected locally)
Blattella asahinai (collected locally) Parcoblatta pennsylvanica (collected locally)
Blattella lituricollis Supella longipalpa (collected locally)

Bed bugs

Our lab maintains several strains of bed bugs, including the Harold Harlan strain, an insecticide-susceptible strain maintained by Dr. Harlan for the past 3+ decades. Bed bugs are maintained completely “in vitro” without use of animal hosts. We use custom-made jacketed glass feeders and rabbit blood. Contact Coby Schal or Rick Santangelo for more information. We gladly share protocols and bugs.

Lab facilities

Our lab is located in Gardner Hall, on the North Campus of NC State. It contains a separate insect rearing room, a microscopy/physiology room, a chemical ecology/biochemistry room and a behavioral assay section, which alleviates problems of cross-contamination with pheromones and hormones. The behavior lab consists of a walk-in temperature and humidity microprocessor-controlled room and 7 reach-in incubators. We are fully equipped for cockroach and bed bug rearing and maintenance.
The Biological Resources Facility houses small animals and generates antibodies on a fee-for-service basis. A hybridoma facility is available at the College of Veterinary Medicine.

Analytical equipment

Equipment in our lab includes:
  • Two HP1050 HPLCs, with diode array detectors (old, but reliable)
  • Bio-Tek PowerWave 200 microplate scanning spectrophotometer (old, but used by many)
  • Agilent 6890 (S/SL, FID) and 7890 (S/SL, on-column, FID) capillary GCs, each with a HP7683 auto-injector
  • Agilent 6890 GC coupled to an Agilent 5975 MSD with both EI and CI capabilities
  • Agilent 7890 GC-EAD system (S/SL, FID) (home made coupling and amplification)
  • HP5890 prep-GC system (S/SL, on-column, FID)
  • P160N, HK160 and MP8-1 digital balances
  • Centra CL5R centrifuge, and several microfuges
  • Zeiss and Nikon SMZU dissecting microscopes with fiber optics
  • Ultrasonic and manual tissue disrupters
  • Bio-Rad electrophoresis systems
  • Brinkman Polytron homogenizer
  • Jouan RCT60 concentrator
  • Several freezers including -80oC freezers
  • Various molecular biology and microbiology equipment
Behavioral equipment includes:
  • Several IR sensitive cameras and high resolution monitors
  • Digital video recorders
  • Noldus’s EthoVision and The Observer packages.